发明名称 |
A MICRODEVICE FOR A FLUORESCENCE-BASED ASSAY, AND A METHOD FOR MAKING THE MICRODEVICE |
摘要 |
<p>Glass as a substrate for fluorescence-based assays is expensive. Disclosed i s a poly(cyclic olefin) alternative to glass that is of comparable sensitivity. More specifically, disclosed is a method of making a microdevice for the immobilization of biomolecules for the purpose of carrying out a fluorescence-based assay, sai d method comprising providing a substrate comprising a poly(cyclic olefin); protectin g the substrate from ultraviolet (UV) light; and subjecting a surface of said UV-protected substrate to ozone oxidation to activate said surface; wherein a content of intrinsic fluorophores on the surface of the substrate remains substantially unchanged after the ozone oxidation. Also disclosed is a microdevice for the immobilization of biomolecules for t he purpose of carrying out a fluorescence-based assay, said microdevice comprising a body comprising a poly(cyclic olefin), said body having an ozone-activated surface substantially free of intrinsic fluorophores; wherein said activated surface is substantially free of intrinsic fluorescence during a fluorescence-based assay of said biomolecules bound to said activated surface.</p> |
申请公布号 |
CA2568904(A1) |
申请公布日期 |
2008.05.23 |
申请号 |
CA20062568904 |
申请日期 |
2006.11.23 |
申请人 |
NATIONAL RESEARCH COUNCIL OF CANADA |
发明人 |
DIAZ-QUIJADA, GERARDO A.;VERES, TEODOR |
分类号 |
C40B60/02;C12Q1/68;C40B60/08;G01N33/50;G01N33/52;G01N33/543 |
主分类号 |
C40B60/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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