发明名称 |
WERKWIJZE VOOR DE BEREIDING VAN EEN PENICILLINE-DEACYLASE BEVATTEND PREPARAAT. |
摘要 |
Prepn. of cell-free penicillin-acylase by fermenting a strain of E. coli which produces this enzyme. The intracellular enzyme is separated from the culture liquid at controlled temps. between 0-50 deg.C by fast removal of the cell material through a slot or opening at a pressure of at least 500 psi but below the pressure which would badly tear the cells. The removed cell material is then stirred in water for 0.1-5 hrs. at 10-50 deg.C to dissolve the enzyme; this is extracted from the inactive cell material in aqs. soln. The cell-free enzymes are used for the prodn. of 6-APA and are esp. suitable for direct removal of side-chains of natural penicillins, esp. the side-chains of benzyl penicillin. The enzymes may be used as starting materials for chemical modification of the enzyme, giving products with higher activity and/or wider technical application. |
申请公布号 |
NL158547(B) |
申请公布日期 |
1978.11.15 |
申请号 |
NL19690002045 |
申请日期 |
1969.02.10 |
申请人 |
AKTIEBOLAGET ASTRA, SOEDERTAELJE, ZWEDEN. |
发明人 |
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分类号 |
C12P37/00;C07D499/00;C07D499/10;C07D499/12;C07D499/18;C07D499/42;C07D499/44;C12N9/84;C12N11/10;C12P37/04;C12P37/06;C12R1/19 |
主分类号 |
C12P37/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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