METHOD OF BONDINE BIOLOGICALLY ACTIVE PROTEINS TO INORGANIC SUPPORT MATERIALS

摘要

1532160 Immobilized enzymes on a waterinsoluble support CORNING GLASS WORKS 23 Sept 1977 [27 Sept 1976] 39716/77 Heading C3H A process for bonding a biologically active protein to an inorganic support material comprises:- (i) treatment of a water-insoluble inorganic support material having a surface area greater than 10 m2/g. and a surface isoelectric point (the pH at which the electrostatic surface potential is zero) at a pH of less than 7À0 with a solution of 1,4-diaminobenzene, whereby the 1,4-diaminobenzene is adsorbed on to the surface of said support material; (ii) separation of said treated support material from the solution; (iii) reaction of said separated treated support material with a solution of a biologically active protein, thereby to covalently bond said biologically active protein to said support material via the amino groups of said 1,4- diaminobenzene. The support material may be siliceous, preferably a glass or silica, or selected from stannic oxide, titania, manganese dioxide or zirconia. The protein solution may be a solution of at least one enzyme, preferably papain, trypsin or lactase. The product of (ii) may be diazotized or subjected to a Schiff base reaction (reaction of the amino group of 1,4-diaminobenzene with an aldehyde), In the Examples, porous glass coated with 1,4-diaminobenzene is (i) diazotized and reacted with papain and trypsin and (ii) treated with glutaraldehyde and reacted with trypsin; porous silica coated with 1,4-diaminobenzene is treated with glutaraldehyde, and reacted with lactase.