ESTABLISHMENT AND USE OF AN IN VITRO PLATFORM FOR EMT

摘要

Exemplary embodiments of the present disclosure are directed towards products that serve as models for epithelial to mesenchymal transition, methods and uses thereof. The product comprises of a plurality of components, wherein the plurality of components are selected from one or more of the following groups: a primary epithelial cell population; a primary fibroblast cell population; a first immortalized stable cell line with epithelial or intermediate phenotype harbouring an engineered immortalizing gene construct; a second immortalized stable cell line with epithelial or intermediate phenotype harbouring an engineered immortalizing gene construct and an engineered assayable marker gene construct; a third immortalized stable cell line with mesenchymal-like or mesenchymal phenotype harbouring an engineered immortalizing gene construct, an engineered assayable marker gene construct and an engineered EMT inducing gene construct; an EMT modulating agent; and an enriched population of cancer stem cells derived from the third immortalized stable cell line.