ISOLATION OF COAGULATION FACTORS FROM BIOLOGICAL MATERIAL

摘要

The blood coagulation factors I (fibrinogen), VIII (antihemophilia factor, abbreviated AHF), and factor IX (also called B-factor) are isolated in high yields from animal tissue materials such as blood or blood products (e.g. plasma) or plasma fractions, or the B-factor is purified from a B-factor concentrate, by a procedure involving the essential step of adsorption (as in affinity chromatography) of at least one of these factors, in a liquid system, onto a water-insoluble gel matrix primarily composed of a cross-linked sulfated or sulfonated, gel-forming carbohydrate such as cross-linked dextran sulfate-agarose, crosslinked dextran sulfate-dextran, cross-linked heparin-agarose and other such gel matrix-providing substances.