| 摘要 |
An affinity chromatography method for the separation of high-activity heparin from a mixture thereof with low-activity heparin. The method employs an affinity column of DEAE-Sephadex and a series of sodium chloride-imidazole elution buffers (pH 6.5-7.5) varying in sodium chloride molarity from about 0.5 to about 1.0. Stepwise elution of a plurality of heparin fractions from the affinity column with the elution buffers of successively increasing sodium chloride molarity results in the removal from the affinity column of first the low-activity heparin and finally the high-activity heparin.
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