PROCESS FOR PREPARING A CURING AGENT FOR LEUKEMIA

摘要

A therapeutic agent for leukemia is prepared by grinding down placenta with water or diluted physiological saline to make an emulsion and adding a mixture of aqueous acetic acid and hydrochloric acid to the emulsion to acidify it to 0.5 to 2.0 N in acidity; heating the acidified emulsion; after cooling, removing insoluble protein by centrifugation and further, after neutralizing with an alkali solution, centrifuging said emulsion to remove insoluble matter and collect a clear supernatant; concentrating said supernatant, and dialyzing the resulting concentrated liquid through a dialysis membrane or filtrating by membrane filter to obtain a dialyzed fluid or filtrate; subjecting the dialyzed fluid or filtrate, after concentrated, to a column chromatography, upon using Sephadex, to obtain any one of fractions, of 0.96 - 1.82 in distribution coefficient with Sephadex G-25, of 0.35 - 1.24 with Sephadex G-15, or of 0.35 1.25 with Sephadex G-10; and lyophilizing the fraction. Sephadex is a three dimensional network structure of dextran cross-linked with epichlorohydrin, which has numerous OH groups in the interior of the network structure, and is strongly hydrophilic so that it swells in water or an electrolyte solution to turn into a gel. The gel particle has the property of restricting a molecule larger than a certain size to get into the interior therein and serves as a kind of sieve. The gel particle is therefore used as a molecular sieve. Sephadex is a trademark of a product sold by Pharmacia Fine Chemicals Company, Sweden.