发明名称 |
VERFAHREN ZUR ELEKTROPHORETISCHEN UNTERSUCHUNG VON PROTEINEN |
摘要 |
<p>In a new procedure for the electrophoretic examination of proteins (enzymes, serum proteins, tissue, homogenates) involving introduction of the proteins by means of a carrier medium into holes in a flat layer of carrier gel (agar, starch or polyacrylamide gel), there is used as the carrier medium a swollen gel (preF. with a low degree of crosslinking) in the form of dextran swollen with water or buffer soln. with the addition of sucrose. Use of sucrose-contg. dextran gel as carrier medium enables protein to be applied to the carrie medium by simple pipetting. the sucrose stabilises the proteins against damage while passing through the dextran. Larger amts. of protein can be used than with known methods, and even minor proteins (e.g. human erythrocyte phospho-glucomutase isoenzyme 3) can be detected.</p> |
申请公布号 |
DE2704096(A1) |
申请公布日期 |
1978.08.03 |
申请号 |
DE19772704096 |
申请日期 |
1977.02.01 |
申请人 |
BISSBORT,SIEGBERT,DIPL.-BIOLOGE |
发明人 |
BISSBORT,SIEGBERT,DIPL.-BIOLOGE |
分类号 |
G01N27/447;(IPC1-7):01N27/26;01N33/16 |
主分类号 |
G01N27/447 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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