| 摘要 |
A determination of the presence and amount of a specific organic substance (Ligand) that will form a complex with a macromolecule (antibody) may be made by means of a reagent obtained by binding to the Ligand to be assayed a chemiluminescent substance. The addition of a limited amount of a substance (antibody) having receptors for the ligand together with the chemiluminescent labeled ligand to the fluid to be assayed results in a competitive reaction between the Ligand present in the fluid and the chemiluminescent labeled Ligand for the limited number of receptor sites. Under equilibrium conditions, the amount of chemiluminescent labeled Ligand bound to the antibody is related to the amount of unlabeled Ligand in the solution being assayed, and is determined by isolating the antibody and measuring its chemiluminescence or by isolating and measuring the amount of free labeled-Ligand remaining.
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