Method of sample introduction in the micro liquid chromatography and the apparatus therefor

摘要

A method of accurate sample introduction, while holding a good reproductivity, into a chromatographic system by means of employing a pulse-motor as a driving source of a syringe for holding the mobile phase, inversely rotating the same pulse-motor by imparting a certain predetermined number of pulses during the sample collecting or picking-up period in order to cause the syringe to make a sucking action, and making the syringe suck certain predetermined amount of the sample from an open end of a mobile phase supplying line which has been disconnected from the column, and an apparatus for actualizing the method.