| 摘要 |
The present invention encompasses a method for stabilizing interferon during purification, concentration, packaging, filtering and storage comprising treating an interferon preparation with an effective stabilizing amount of reduced glutathione, thiodiglycol, thioethanolamine, thioalkanoic acid having 1-7 carbon atoms, monothioglycerol, dithiothreitol, thioctic acid, N-acetylcysteine, or N-acetyl-homo-cysteine, as a sulfur containing reducing agent for selectively retaining interferon thiols in the reduced state. The method of the present invention preserves the activity of interferon by selectively retaining interferon sulfhydryl (SH) functions in the reduced state without reducing interferon disulfide linkages (-S-S-) which are essential for activity, or by selectively blocking interferon sulfhydryl groups.
|
