POTENTIAL SENSING CELL ANALYZER

摘要

The disclosure relates to an apparatus and method for electrically analyzing biological cells. An orifice having two potential sensing electrodes is immersed in a container of saline solution in which cells to be analyzed are suspended. The entrance of the orifice communicates with the saline solution and the exit of the orifice communicates with a device for evacuating the cell containing saline solution through the orifice. Two potential sensing electrodes embedded in the orifice are connected to a high impedance differential amplifier which outputs to a pulse-height analyzer and a display or recording device. In operation, a source of current is applied to the orifice through external power electrodes to provide an electrical potential across the electrodes. Cells within the solution passing through the orifice vary the resistance between the electrodes and hence the potential difference between the electrodes. The high impedance differential amplifier amplifies this change in potential difference so that it can be received by the pulse height analyzer and recorded or displayed.