| 摘要 |
<p>An ion exchange resin carries the enzyme glucoamylase, which is quasi-irreversibly fixed to it by physical adsorption, without denaturing on the resin. The resin is pref. a crosslinked polymer of styrene with divinylbenzene, esp. 'Amberlite' (RTM), e.g. 'Amberlite IRa 93'. The resin pref. contains 0.05-2.5 ml glucoamylase per g of dry resin, partic. 0.25ml or 0.75ml. Prepn. of the resin is effected by placing it in contact with a buffered glucoamylase soln. for 6-24 hrs. at 1-10 degrees C. The resin is pref. first dried at about 17.5mm Hg pressure. The buffered soln. pG is pref. 3-7. The prepd. resin can be used to hydrolyse maltose, dextrin or starch solsn. at 10-50 (pref. 40) degrees C. The maltose and dextrin solns. are pref. 10-50% w/v, and the starch soln. 1-10% w/v. Such solns. are pref. first palsteurised before hydrolysis. Glucoamylase cannot be fixed by procedures hitherto used for different types of enzymes.</p> |
