| 摘要 |
1514192 Immunological test device GENERAL ELECTRIC CO 27 March 1975 [1 April 1974 (3)] 12834/75 Heading G1B Apparatus for detecting immunologically reactive substances comprises a solid substrate member having a metallized surface area, and means overlying the substrate member for locating a thin layer of liquid on the metallized surface. The substrate member may be of glass, metal or plastics. The metallized layer may be a non-continuous layer of indium globules of average thickness 2000 to 4000A, a continuous film of a single metal or of an alloy of two metals such as indium and gold on a layer of a single metal or an alloy of two metals with an oxide film of one of the metals, for example indium/indium oxide, nickel/nickel oxide, gold/indium alloy/indium oxide. The means for providing an immobilized layer of moisture may be a layer of gel less than 1 mm thick, a cellulose or cellulose acetate membrane or a filter paper or tissue, or alternatively a glass cover member may be applied to the surface of the substrate member and moisture introduced to the area around the inner edges of apertures formed in the cover member or along edges of the cover member by introduction of water into the apertures. In the embodiment of Figs la, 2b, a dish with a metallized upper surface 11 contains agar or agarose gel 12 having wells 13a to 13e formed through it, and immunologically reactive components placed in the wells, for example in 13a, 13c respectively diffuse outwardly forming a precipitin area 14 which remains for inspection on the metallized surface of the dish when the gel layer has been removed. In the embodiment of Fig 11B, a monomolecular layer 10c of one immunologically reactive component is formed on the metallized surface 10b of the substrate 10, one or more drops of water are applied to the edges of cover member 11 so that an immobilized moisture layer is formed between members 11 a and 11 and one or more drops of a solution of the other of the immunologically reactive components is placed at the moistened edge of member 11 and forms a second monomolecular layer 11a' the width of which is said to be a measure of the concentration of the latter component. The application of the second immunologically reactive component solution through an apertured cover plate may take place under pressure through an L-shaped tubing inserted into one of the apertures Figs 11A to 12B (not shown), when the solution may flow through a channel (60) formed in the underside of the cover member. The spreading of the solution of the second component may be speeded by application of electrodes at opposed ends of such a flow-path.
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