| 摘要 |
A specific binding assay method and test kit for determining a ligand, particularly an antigen or hapten, in, or the ligand binding capacity of, a liquid medium, particularly a body fluid such as serum, wherein the liquid medium is combined with assay reagent means including a labeled binding component such as a radiolabeled form of the ligand, with the improvement that separation of the resulting bound species and free-species of the labeled component is accomplished by selectively adsorbing the free-species onto a solid comprising a nonion-exchange cross-linked polyvinyl alcohol. The method is particularly suited to the radioimmunoassay detection of nonproteinaceous haptens such as thyroxine, triiodothyronine, estriol, vitamin B12 and digoxin. The polyvinyl alcohol adsorbent provides a very effective and readily available separation means for use in specific binding assays. |
