Stabilising protein during separation, purification and storage - by adding amphiphilic cpds., esp. lipid(s), ionic and or nonionic detergents

摘要

Stabilisation of sensitive proteins and related cpds., esp. lipo-proteins and glyco-proteins, during their purification, sepn. and storage, is effected by adding protective substances, (I), having an amphiphilic structure, pref. lipids, partic. phospho-lipids, ionic and/or nonionic detergents. In an example, Ca2+-adenosine triphosphatase of human erythrocyte membrane was separated by gel chromatography in the presence of lecithin and "Tween 20" (RTM).