KOSOOMOCHIITAKOGENKETSUTEIKIGUJUBUTSUSHITSUNOSOKUTEIHOHO

摘要

PURPOSE:To simplify the operation with a higher sensitivity by making a bond of materials having at least one antigen determinant in common in a sample and a specified enzyme react with an antibody together with the sample to measure the activity of the enzyme. CONSTITUTION:A material 1 having an antigen determinant contained in a sample such serum and a material 2 having antigen determinants at least one of which is in common to the material 1 are bonded to an enzyme such as amilase allowed to act on a high molecular substance such as water insoluble and soluble dextran and an antibody to react with the common antigen determinant in the solution is made to react with the materials 1 and 2 by contact. Thereafter, the high molecular substance is brought into contact with the bond for oxygen reaction and the activity of the enzyme is measured depending on the decrease in the high molecular substance.