Novel means and method for diagnostic quantitation of serum or plasma bilirubin

摘要

Method of determining total and direct bilirubin in blood serum or plasma. A first reagent containing sulfanilic and hydrochloric acids and a second reagent containing sulfanilic acid, hydrochloric acid and dimethyl sulfoxide are disposed in a scheme of reagent reservoirs. Into some of the reservoirs is also placed sodium nitrite contained in frangible capsules. The frangible capsules are broken with the resulting formation of a diazonium reagent. Blood serum or plasma, standard bilirubin solution and control serum are added to the reservoirs according to the analytical scheme. The subsequent color developments in the reservoirs are correlated to the bilirubin contents.