| 摘要 |
<p>Dehydrated or rehydratable agarose or agar-agar gels is prepd. by incorporating antibodies immobilised by ion exchange on a weakly basic polymer (I) present in the gel network. The gel may be derived from normal or high-purity agarose, agarose with high electroendo-osmotic props. or agar-agar. (I) is pref. "Separan CP-35" (RTM: for a polyacrylate) or a semisynthetic material such as diethylaminoethyl (DEAE)-dextran; a heteropolymer made by polymerisation of acrylamide in DEAE-dextran soln., or a copolymer of acrylamide with DEAE-dextra. PRef. the gels are cast as films on a polyester support, esp. "Scotch-par L-13" or "Cronar Clear Base C-72" (RTM's). Used in determination of serum proteins by e.g. radial immunodiffusion, electro-immunodiffusion or two-dimensional immunoelectrophoresis. Tendency of the antibody to migrate in the dehydrated gel is eliminated, but when the gel is rehydrated the antibody is uniformly distributed and the product is equivalent to a fresh gel. After use the antibody is eliminated by washing with 0.9% NaCl soln.</p> |
