发明名称 |
Production of viruses in tissue culture without use of serum |
摘要 |
Growth of cells in tissue culture and virus vaccine production in tissue culture in the absence of serum are disclosed. The cells in tissue culture are grown initially in a chemically defined medium containing protamine zinc insulin, and the protamine zinc insulin medium is removed before virus propagation. Supplementation with serum or serum fractions can be eliminated entirely; and improved virus production in a serum free system can be obtained.
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申请公布号 |
US4072565(A) |
申请公布日期 |
1978.02.07 |
申请号 |
US19740520574 |
申请日期 |
1974.11.04 |
申请人 |
THE DOW CHEMICAL COMPANY |
发明人 |
WEISS, STEFAN ADAM;TORNEY, HARRY LESTER;CALDWELL, WILLIAM JOSEPH |
分类号 |
A61K39/12;A61K39/165;A61K39/20;A61K39/205;C12N5/00;(IPC1-7):C12B3/00;C12K9/00 |
主分类号 |
A61K39/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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