发明名称 METHOD AND APPARATUS FOR DETECTION AND PURIFICATION OF PROTEINS AND ANTIBODIES
摘要 <p>In carrying out a purification and/or concentration step of antigens or antibodies, a substrate is immersed in a first aqueous medium containing a specifically reacting antigen to coat said substrate with a monomolecular layer of said specifically reacting antigen. The resulting coated substrate is then immersed in a second aqueous medium containing immunologically reactive antibody specific to the antigen in the first aqueous medium to complex said immunologically reactive antibody with said specifically reacting antigen. The resulting substrate is then immersed in a reagent capable of cleaving the immunological bond between said immunologically reactive antibody and said specifically reactive antigen and forming a solution of said immunologically reactive antibody in the immunological bond-cleaving solution and leaving said specifically reacting antigen coated on said substrate. The method can be reversed for preparing a purified concentration of an immunologically reactive antigen whereby a specifically reacting antibody is substituted for the antigen and the corresponding immunologically reactive antigen is substituted for the antibody.</p>
申请公布号 CA1025772(A) 申请公布日期 1978.02.07
申请号 CA19730172639 申请日期 1973.05.29
申请人 GENERAL ELECTRIC COMPANY 发明人 GIAEVER, IVAR
分类号 G01N33/531;A61K38/00;A61K39/00;C07K1/14;C07K1/16;C07K16/00;G01N33/543;G01N33/553;(IPC1-7):61K39/02 主分类号 G01N33/531
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