Oligonucleotide multiple reporter precursors

摘要

Oligonucleotides containing multiple free amino groups attached directly to the phosphorus atoms of the phosphodiester linkages having the structure shown below. <IMAGE> wherein D=a ribonucleoside or deoxyribonucleotide; p=a 5' to 3'-phosphodiester or phosphoramidate linkage; n=1 to 20; a=1 to 20; b=1 to 20; c=1=20; z=0 to 20; t=1 to 100; and wherein for each repeating unit, "a" represents the same number or a different number than that number represented by "a" in every other repeating unit; and for each repeating unit "b" represents the same number or a different number than represented by "b" in every other repeating unit. Multiple reporter molecules can be incorporated into the oligonucleotide via the free amino groups allowing for increased probe sensitivity.