发明名称 |
PURIFICATION OF TUBERCULOPROTEINS |
摘要 |
<p>An aqueous solution in a neutral medium of the tuberculoprotein C is subjected to the action of an ionized substance before carrying out three different separation processes which consist in bringing the tuberculoprotein C into solution in water in a proportion of 5% by weight and in neutralizing. The first process consists in adding the ionized substance in an alcohol medium and in carrying out ultracentrifugation for two hours at 40,000 g in order to obtain tuberculoproteins CA in the form of a supernatant liquid. The second process consists in adding a solution of the ionized substance, in carrying out ultrafiltration, concentration and precipitation with trichloroacetic acid in order to obtain the tuberculoprotein CB. The third process consists in adding a solution of the ionized substance then gel-filtering in order to eliminate the molecules having a molecular weight above 200,000 g and in precipitating with trichloroacetic acid in order to obtain a tuberculoprotein CC.</p> |
申请公布号 |
CA1024510(A) |
申请公布日期 |
1978.01.17 |
申请号 |
CA19740200710 |
申请日期 |
1974.05.23 |
申请人 |
INSTITUT PASTEUR |
发明人 |
AUGIER, JACQUES;AUGIER-GIBORY, SOLANGE |
分类号 |
G01N33/569;A61K39/04;A61K39/40;A61K49/00;C07K1/30;C07K1/34;C07K14/005;C07K14/195;C07K14/35;C07K14/41;(IPC1-7):01N33/16 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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