| 摘要 |
1496393 Phosphorous determination PIERCE CHEMICAL CO 17 April 1975 [23 April 1974] 15799/75 Heading G1B An aqueous solution comprises a reducing agent for reducing a phosphomolybdate complex to form molybdenum blue colour and a water-soluble hydrocarbon amine/ethylene oxide adduct, or a quaternary derivative thereof, which is cationic in acid solution and contains lipophilic and hydrophilic non-ionic groups, the adduct or quaternary derivative thereof being present in an amount sufficient to prevent protein precipitation when the solution contains a protein, molybdic acid and a phosphomolybdate complex. The adduct or quaternary derivative thereof preferably has the Formula wherein X<SP>m</SP> - is a non-interfering anion which does not effectively compete with phosphorous for molybdenum or does not promote interference with the blue colour of a phosphomolybdate complex; m is 1, 2 or more; R is a lipophilic group containing from 6 to 28 carbon atoms; R' is H or -CH 2 -CH 2 -(OCH 2 CH 2 ) x -O-CH 2 -CH 2 OH; R" is H or a lipophilic group containing from 1 to 10 carbon atoms; x and y are integers with the sum of x and y being from 5 to 30; and n is 0 or 1. The aqueous solution may be used in the spectrophotometric determination of inorganic phosphate in samples such as sera and urine. |
