| 摘要 |
Analytical multi-spectral optical detection systems and methods. A light source provides one or multiple lines (e.g., discrete wavelengths) of high spectral purity excitation light that is optically coupled to a sample via delivery fiber optic cables. Emission light is collected and provided to an emission detector, such as a diffraction gradient spectrophotometer emission detector, using collection fiber optic cables bundled with the delivery fiber optic cables in a probe interface positioned proximal a sample holder. The probe interface may be scanned over one or more samples, or one or more samples may be scanned proximal a fixed interface probe. Multiple excitation wavelengths allows for simultaneous excitation and detection of multiple fluorescent dyes in the visible spectrum. This increases sample throughput and reduces signal variations associated with signal acquisition at different times. The optical system provides several advantages over other systems including higher sensitivity, improved compatibility with fluorescent dyes, better signal discrimination, increased system reliability and reduced manufacturing and service costs.
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