PROCESS FOR PRODUCING A BINDER FACTOR FOR FOLIC ACID

摘要

There is provided a rapid and less costly radioisotopic assay for measuring the concentration of folate in blood serum. This procedure utilizes 3H-pteroylmonoglutamate, unlabeled 5-methyl-tetrahydrofolic acid, and a partially purified folate binder, such as for example a folate binder extracted from hog kidney. The procedure involves radioisotopically relating the bound amounts of a labeled folate and a known folate at various concentrations of the known folate in a system containing a predetermined amount of the labeled folate, a predetermined amount of the binder factor for the folates, and a predetermined amount of defolated test serum.