Method and system for sample preparation

摘要

A method for preparing a sample by utilizing a shearing force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. Once nucleic acid molecules are obtained, magnetic nanoparticles are used to concentrate and clean the nucleic acid molecules for further testing.

主权项

1. A disposable cartridge for preparing a nucleic acid sample, the disposable cartridge comprising:
a cartridge body with an inner cylindrical surface; a cylindrical insert disposed within the inner cylindrical surface of the cartridge body rotatably connected thereto, the cylindrical insert comprising:
a disrupting chamber fluidly connected to a first port;at least a second chamber fluidly connected to a second port; andfirst and second elongated channels disposed on opposite radial positions of a bottom edge of the cylindrical insert, each traversing at least a portion of the bottom edge of the cylindrical insert, the first elongated channel being fluidly isolated from the second elongated channel, the first elongated channel being fluidly connected to a first pair of channel ports disposed at a first radial position and the second elongated channel being fluidly connected to a second pair of channel ports disposed at a second radial position.