| 摘要 |
PROBLEM TO BE SOLVED: To provide methods and compositions related to improved bacteria-mediated plant transformation.SOLUTION: The invention provides a method for transforming a plant cell with reduced vector backbone integration and a high frequency of low-copy transformation events, which method comprises contacting a plant cell with a bacterial cell that is competent for the transformation of the plant cell, where the bacterial cell comprises a DNA construct comprising: i) at least one T-DNA border region; ii) at least one heterogeneous transgene adjacent to the border region; iii) a coding region for a bacterial selection marker; and iv) at least one segment of DNA comprising cis and/or trans element(s) of repABC replication origin, and selecting a transformed plant cell on the basis of the presence of at least one heterogeneous transgene from the DNA construct and the absence of the vector backbone DNA.SELECTED DRAWING: None |
