METHODS AND APPARATUS TO SEQUENCE A NUCLEIC ACID

摘要

Methods, systems, apparatus and machine readable media are disclosed to sequence nucleic acid. An example method includes subjecting a sequence of target nucleotide bases captured on a microtransponder to a plurality of sequencing reactions to build a sequence of labeled nucleotide bases that are complementary to and bound to the sequence of target nucleotide bases. The example method also includes identifying each labeled nucleotide base of the sequence of labeled nucleotide bases and each respective complementary target nucleotide base of the sequence of target nucleotide bases to which the labeled nucleotide base is bound after each sequencing reaction. In addition, each labeled nucleotide base of the sequence of labeled nucleotide bases and each respective complementary target nucleotide base of the sequence of target nucleotide bases to which the labeled nucleotide base is bound is associated with a microtransponder identification number.

主权项

1. An apparatus comprising:
a microtransponder having a surface to which a nucleic acid target is to be captured via a capture probe; a reaction station to subject the surface-bound nucleic acid target to a plurality of sequencing reactions to add a sequence of nucleotide bases that are complementary to and bound to the sequence of target nucleotide bases; a wash station to remove an extraneous sequencing reagent resulting from the plurality of sequencing reactions; and an identification station to identify each added nucleotide base after each sequencing reaction.