| 摘要 |
<p>Measuring the content of a specific, glycine-conjugated bile acid selected from cholylglycine (I), chenodeoxycholylglycine (II), deoxycholylglycine (III) and sulpholithocholylglycine (IV) in a sample comprises (a) providing an antiserum which is specific for one of the glycine-conjugated bile acids. Process further comprises (b) mixing the sample with the antiserum and with a tyramine deriv. of the same glycine-conjugated bile acid, the tyramine deriv. being labelled with a labelling agent; (c) incubating the mixt. so that the antibodies of the antiserum are bound to the glycine-conjugated bile acid of the sample and the labelled glycine-conjugated bile acid tyramine deriv. Process further comprises (d) the bound antibodies and the unbound material into separate fractions; and (e) measuring the radioactivity of one of the sepd. fractions the amt. of the specific bile acid conjugate being determined by comparison with standard samples. Sulpholithocholylglycyltryamine (IVa), chenodeoxycholylglycyltyramine (IIa), cholylglycyltyramine (Ia) and deoxycholylglycyltyramine (IIIa) are claimed. Process is used in the determn. of the ratio of prim. to sec. bile a acid conjugates, which in turn is useful in the diagnosis of hepatic damage due to infection or chemicals. Method can be used directly on serum sample, and can be carried out directly on analytical instruments without special solns. or ampoules.</p> |
