| 摘要 |
1507810 Determination of ethanol in body fluid CHEMBRO HOLDINGS (PTY) Ltd 12 Jan 1977 [15 Jan 1976] 01070/77 Heading G1B Ethanol in a body fluid is determined by a method comprising providing a predetermined volume of body fluid, oxidizing ethanol in the body fluid using an alcohol oxidase in buffer solution in the presence of excess molecular oxygen and measuring the rate of oxygen consumption using for example an oxygen electrode, the oxidation taking place in the presence of an agent operative to suppress the formation of free oxygen by decomosition of peroxide. The agent may be a peroxidase together with o-tolidine, o-dianisidine, aminoantipyrine, or alternatively a catalase inhibitor such as sodium azide. The alcohol oxidase may be obtained from Kloeckera yeast or from a Basidiomycete. The buffer may be a 0À01 to 2 m potassum phosphate buffer pH 7 to 9, and the reaction mixture may contain 10 microns to 10 mm of a metal ion complexing substance such as EDTA-Na. |
