Frequency domain discrimination and counting technique

摘要

An apparatus and method for counting the number of objects-of-interest present in a selected sample by scanning the sample with a rotating reticle of preselected design to measure the spatial frequency components in the field-of-view, which components are definitive of the number of objects-of-interest present. The spacing within the reticle pattern determines the size of the objects that will be counted. The invention has significant application as a means for counting bone marrow granulocyte precursor colonies in the detection and treatment of leukemia.