ENZYME PARTICIPATING IN C-TERMINAL AMIDATION, AND METHOD OF PREPARING SAME AND USE THEREOF

摘要

ENZYME PARTICIPATING IN C-TERMINAL AMIDATION, PROCESS FOR PREPARING AND USE OF THE SAME An enzyme participating in C-terminal amidation which acts on a peptide C-terminal glycine adduct represented by the following formula (I): (I) (wherein A represents a residue other than .alpha.-amino group or imino group and .alpha.-carboxylic group derived from naturally occurring .alpha.-amino acid, X represents hydrogen atom or a residue of an amino acid derivative which is bonded to N atom through carbonyl group) to form a peptide C-terminal .alpha.-hydroxylglycine adduct represented by the following formula (II): (II) (wherein A and X have the same meanings as above, and an enzyme participating in peptide C-terminal amidation of a peptide terminal glycine adduct which acts on a peptide C-terminal .alpha.-hydroxylglycine adduct represented by the above formula (II) to form a C-terminal amidated compound represented by the following formula (III): (III) (wherein A and X have the same meanings as described above) a method of producing the same, and the use thereof.