| 摘要 |
<p>1526146 Pipette apparatus UNION CARBIDE CORP 15 June 1976 [16 June 1975] 24745/76 Heading G1B Apparatus for loading liquid materials into a rotatable disc having a plurality of cavities adapted to contain liquid and arranged in a plurality of radially aligned rows said apparatus comprises: (i) a first horizontally disposed cam-shaft, (ii) a second horizontally disposed cam-shaft arranged parallel to and horizontally separated from said first cam shaft, (iii) electrically actuatable motor means engaged to said first and second cam-shafts, (iv) a first vertically disposed shaft arranged at a position substantially in-line with the axis of rotation of said second cam-shaft and spaced adjacent from said first cam-shaft, said first vertically disposed shaft being rotatably moveable about its vertical axis, (v) a disc member removably affixed to said vertical shaft and rotatable therewith, said disc member having a plurality of cavities adapted to contain liquid, said cavities being arranged in a plurality of equi-spaced radial rows, (vi) reservoir means adapted to contain liquid reagent positioned adjacent said disc member, (vii) an annular ring member fixedly positioned with respect to and surrounding said disc member, said ring member having a plurality of receptacles adapted to contain serum, said receptacle being in radial alignment with said radial rows of said disc member, (viii) a second vertically disposed shaft arranged adjacent said first vertically disposed shaft, said second vertically disposed shaft being reciprocally rotatably moveable about its vertical axis and being vertically moveable up and down, (ix) a first probe supporting arm fixedly attached to said second vertically disposed shaft, said first probe supporting arm being arranged such that a probe supported thereon can be moved from a position above said reagent liquid reservoir to a position above an initial selected radial row of said disc member and back to a position above said liquid reagent reservoir upon reciprocal rotatable movement of said second vertically disposed shaft, (x) a first cam mounted on said first cam-shaft and engaged to said second vertically disposed shaft to lower and raise said shaft and said first probe supporting arm when the probe supported by said arm is positioned over said reagent reservoir to cause said probe, when lowered, to be immersed in liquid reagent in said reagent reservoir and to subsequently lower and raise said first probe supporting arm when the probe supported by said arm is positioned over said initial selected radial row of said disc member to cause said probe, when lowered, to be within a cavity of said selected radial row, (xi) a second cam member mounted on said first cam-shaft and engaged to said second vertically disposed shaft to reciprocally rotate said second vertically disposed shaft and said first probe supporting arm from an initial position in which the probe supported by said arm is above said liquid reagent reservoir to a second position in which said probe is above said initial selected radial row of said disc member and back to its said initial position, (xii) a horizontally, moveably mounted block arranged above said second cam-shaft, (xiii) a second probe supporting arm pivotally mounted on said horizontally moveable block and arranged to be rotatably moveable up and down in a vertical plane, (xiv) a rinse liquid reservoir arranged adjacent said second cam-shaft, (xv) a third cam mounted on said second camshaft and engaged to said horizontally moveable block to move said second probe supporting arm such that the probe supported thereby is moved in sequence from (a) an initial position in said rinse liquid reservoir to (b) a position within a said receptacle of said ring member to (c) a position within a cavity of a radial row of said disc member and (d) back to its initial position within said rinse liquid reservoir, (xvi) a fourth cam mounted on said second camshaft and engaged to said pivotally mounted second probe supporting arm such that the probe supported thereby is raised during sequence (a) of (xv) above, lowered during sequence (b) of (xv) above, raised subsequent to sequence (b), lowered during sequence (c) of (xv) above, raised subsequent to sequence (c) of (xv) above, and lowered during sequence (d) of (xv) above, (xvii) first syringe pump means arranged adjacent said first cam-shaft and communicating with a said probe supported by said first probe supporting arm, (xviii) fifth cam means mounted on said first cam-shaft and engaged to said first syringe pump means to cause relative movement of the syringe of said first syringe pump means such that liquid reagent is drawn into a said probe of said first probe supporting arm when said probe is immersed in liquid reagent in said reagent reservoir and dispensed from said probe when said probe is within a cavity of a selected radial row of said disc member, (xiv) second syringe pump means arranged adjacent said first cam-shaft and communicating with said probe supported by said second probe supporting arm, (xx) sixth cam means mounted on said first camshaft and engaged to said second syringe pump means to cause relative motion of the syringe of said second syringe pump means such that serum is drawn into said probe of said second probe supporting arm when said probe is within a said receptacle of said ring member and dispensed from said probe when said probe is within a cavity of a radial row of said disc member, (xxi) seventh cam means mounted on said first cam-shaft and engaged to said first vertically disposed shaft to rotate said shaft by an increment corresponding to one radial row of cavities of said disc member subsequent to the dispensing of liquid from the probes supported by said first and second probe supporting arms. A suitable form of apparatus is shown in Fig. 1 and comprises a removeable multicavity transfer disc 67 and sample ring 69. Upon actuation of appropriate panel switches, a precise amount of liquid reagent is drawn into the reagent probes 34 and 35 from reservoir 50 and the reagent arm 31 raised and swung over to extend probes 34 and 35 into cavities of a radial row 5 of disc 67. Reagent is then dispensed into the cavities and reagent arm raised and moved back to its initial position. Concurrently, sample arm 55 and sample probe 62 is moved from wash liquid reservoir 66 to the sample intake position where probe 62 is immersed in the liquid in sample cup 68 and a precise amount of sample drawn into probe 62 which is then moved to the sample dispense position and the amount of sample dispensed into an appropriate cavity of a radial row in disc 67. The sample arm 55 and probe 62 are returned to the starting position. This intakedispense procedure is repeated for each cavity row to be filled, the disc 67 and ring 69 being advanced after each intake-dispense cycle to bring the next cavity row into position. The above movements of arms, transfer disc and sample ring is derived from shafts connected to a suitable motor means, and the movements controlled by cams mounted upon these shafts.</p> |
