Treatment of cell culture microcarries

摘要

A method of treating certain cell culture microcarriers to improve their performance is disclosed. In this method, positively charged microcarriers, such as those produced by reacting polydextran beads with diethylaminoethyl, are treated by contacting them with macromolecular polyanions, such as carboxymethylcellulose, prior and/or during use in cultures. Such treatment overcomes deleterious effects previously observed in attempts to use these microcarriers in cell culture systems.