PREPARATION OF STERILE CULTURE MEDIA

摘要

1478238 Preparation of sterile culture media ISORAD ISOTOPES & RADIATION ENTERPRISES Ltd and STATE OF ISRAEL REPRESENTED BY THE ATOMIC ENERGY COMMISSION 15 May 1975 [17 May 1974] 20700/75 Heading C6F In a process for preparing by ionizing irradiation a sterile culture medium substantially biologically equivalent to the untreated culture medium, the culture medium is first augmented with sufficient quantities of any components which are partially destroyed by the irradiation e.g. indicators and vitamins, or with radio-protectors. Catalase may additionally be included to prevent the formation of toxic degradation products. The irradiation may be gamma radiation at a dose of 0À5 to 2À5 Mrad, x-rays or electron beams of suitable intensity. The medium may be poured into plastics Petri dishes and these packaged in polyethylene sleeves prior to the treatment. Examples disclose (1) triple sugar iron agar with additional phenol red indicator, (2) Mueller Hinton agar with catalase and an increased content of agar, (3 and 4) fluid thioglycollate medium containing additional thioglycollate or cysteine, (5) MacConkey agar with augmented neutral red indicator and containing additionally thioglycollate or cysteine, (6) a lactose agar medium with augmented bromothymol blue indicator and containing additionally thioglycollate or cysteamine, and (8) tryptose agar with an increased content of agar. Example 7 discloses a plastics slide coated on its two sides with the modified agar media of Examples 5 and 6, respectively.