UREA ASSAY PROCESS AND COMPOSITION

摘要

Assay of urea is provided by spectrophotometric measurement of a test solution containing the urea to be assayed together with ATP: urea amido-lyase and adenosine triphosphate as co-substrate for the enzyme, together with nicotine adenine dinucleotide, reduced; phosphoenol pyruvate; lactate dehydrogenase; pyruvate kinase; and magnesium and potassium ions. The transformation of the urea is followed quantitatively by optical absorption measurements at 340 millimicrons. The assay is unaffected by the presence of non-urea nitrogen, such as ammonia.