摘要 |
1474418 Cellular membranes KERNFORSCHUNGSANLAGE JULICH GmbH 22 May 1974 [23 May 1973] 22965/74 Heading B1X [Also in Divisions C2 and C3] Vesicles with semi-permeable membrane walls are obtained from cells of an organism, e.g. erythrocytes, and used as carriers for enzymes. The enzyme is foreign to the cell and is inserted into the vesicle by osmosis from a solution having a lower osmolarity than the cell contents. The entrapped enzyme may then be used to carry out biochemical synthesis or degradation reactions involving the enzyme by treating solutions of the starting materials having osmolarities sufficiently similar as to maintain the cell membrane impermeable to the enzyme, whereby the starting matterials may diffuse through the membrane, undergo reaction, and the products then diffuse out. In the example invertase is entrapped and used to convert cane sugar to glucose and fructose. |