| 摘要 |
An improved chromogenic substrate for alpha -amylase assays is synthesized by reacting amylose with Cibachron Blue F3GA, sodium sulfate and trisodium phosphate and incubating the resultant dyed amylose substrate with a buffer containing calcium chloride. The new substrate is more sensitive than commercially available chromogenic substrates in determining the alpha -amylase activity of the enzymes of sweet potato, tobacco, and human saliva.
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