发明名称 |
Protein-assay reagent and method |
摘要 |
A dye binding reagent for protein assay comprises Coomassie Brilliant Blue G-250 and an acid having a pKa of from 1 to 2. Upon addition of the reagent to a protein-containing solution, the attendant color change can be spectrophotometrically measured to quantitate even micrograms of protein. Furthermore, the reagent is applicable to a wide range of proteins and requires only about 2 minutes for measurement. Addition of an alcohol such as ethanol to the reagent further improves sensitivity.
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申请公布号 |
US4023933(A) |
申请公布日期 |
1977.05.17 |
申请号 |
US19760694668 |
申请日期 |
1976.06.10 |
申请人 |
THE UNIVERSITY OF GEORGIA |
发明人 |
BRADFORD, MARION M.;WILLIAMS, WILLIAM L. |
分类号 |
G01N33/68;(IPC1-7):G01N33/16 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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