| 摘要 |
1471329 Fermentation processes ASINOMOTO CO Inc 4 Sept 1974 [4 Sept 1973] 38717/74 Heading C2C [Also in Division C6] An N-acyl derivative (as defined below) of glutamic acid, glutamine or homocysteic acid, or non-bactericidal salt thereof, is added to a bacterial fermentation medium either before or during culturing of bacteria therein, whereby to inhibit bacteriophage activity. According to the invention the N-acyl group has at least 13 carbon atoms, and the carbon chain of the N- acyl group is straight and unbranched, saturated or unsaturated. Preferred compounds are the arachidoyl, myristoyl, oleoyl, palmitoyl and stearoyl derivatives of glutamic acid, glutamine and homocysteic acid, including the sodium or potassium salts. The compound may be added to the fermentation medium in an amount ranging between 0À1 and 200 Ág/ml. Fermentation processes for the production of antibiotics, enzymes, amino acids, nucleotides and nucleosides are mentioned. Examples 1 to 7 relate to the microbiological production of glutamic acid by cultivation of Brevibacterium lactofermentum ATCC 13869. For experimental purposes the culture medium contained in certain cases known amounts of specified phages. Comparative runs involved the addition to the culture medium of (1) the lauroyl derivatives and (2) derivatives based on the following amino acid moieties: aspartic acid, leucine, lysine, methionine, phenylalanine, proline and valine. Example 7 relates to the production of L-lysine by cultivation of Brevibacterium lactofermentum FERM-P 1711. Examples 8 and 9 are directed to the production of guanosine and protease using Bacillus subtilis FERM-P 2107 and Bacillus subtilis FERM-P 305, respectively.
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