FORFARANDE OCH REAGENS FOR BESTEMNING AV HALTEN ALKALISK FOSFATAS I KROPPSVETSKOR

摘要

A method of measuring the alkaline phosphatase level in a body fluid such as serum or plasma comprising hydrolyzing excess monophosphate ester dissolved in a suitable buffer by action of alkaline phosphatase in a predetermined volume of the body fluid to yield an oxidizable substrate, oxidizing the substrate with excess molecular oxygen in the presence of excess oxygenase and measuring the rate of oxygen consumption, preferably by means of an oxygen electrode. The invention also provides a reagent for use in this method which comprises a diethanolamine-HCl buffer having a molarity of 0.1 M to 4.0 M and a pH of 8.6 to 10.8 and containing magnesium ions in a concentration of 100 to 1000 mu M and an excess of oxygenase.