| 摘要 |
PURPOSE:To easily search the optimum separation condition of a decomposed substance, by arranging a hydrogen ion densitometer (pH meter) to the fundamental constitution of a liquid chromatograph and monitoring a change in the pH of an eluate eluted in a gradient manner. CONSTITUTION:Eluates 1, 2, 3 flow through a pre-mixing tank 6 and a mixing tank 9 through solenoid valves 4, 5, 7 and further flow through a pH value detection part 17 provided with a pH meter and a flow passage change-over valve 10 to reach a liquid feed pump 11. The eluate mixture is sent to a pressure detection part 12, a sample injection valve 13, a separation column 14, a visible ultraviolet part absorptiometric detection part 15 and a pH detection part 18 by the liquid feed pump 11. The pH detection part 17 monitors whether the eluate composition mixed by the mixing tank 9 is proper to electrically or artificially control the eluate composition. The pH detection part 18 monitors pH value at the point of time when the decomposed component concerned is eluted to make clear a pH region permitting the elution of said decomposed component. |
