| 摘要 |
Unsaturated iron binding capacity of transferrin, a serum protein, is determined by adding an iron solution containing a known quantity of excess iron to the serum to saturate the iron binding capacity of the serum transferrin, removing the remaining surplus iron with an absorbent and making a colorimetric determination by causing iron to be released from transferrin under an acidic condition. Since the iron solution is unstable in alkaline zone, it is stabilized by forming it into nitrilotriacetic acid iron chelates. The stability of iron chelates of sodium bathophenanthroline sulfonate is greater than that of nitrilotriacetic acid. This fact is utilized, and the surplus quantity of nitrilotriacetic acid chelates remaining after the saturation of the iron binding capacity of tranferrin is measured by using sodium bathphenanthroline sulfonate. The sodium bathophenanthroline sulfonate iron chelates have maximum absorbance at the wavelength of 535 nm, and Beer's law holds for an iron concentration range up to 600 mg/100 ml. |
