发明名称 |
One pot synthesis of oligosaccharides using multiple enzymes and cmp-sialic acid regenerating system |
摘要 |
A single reaction vessel process for the synthesis of a sialylated galactoside is disclosed. The synthesis utilizes a beta -galactosidase to catalyze the reaction of a galactose-containing substrate and an acceptor to form a new galactosyl glycoside that is then sialylated using a cyclic multienzyme synthesis system to form CMP-sialic acid that sialylates the formed galactosyl glycoside in the presence of an alpha -sialyltransferase. The value of Km/Vmax for the formed galactosyl glycoside as a substrate for the alpha -sialyltransferase is less than one-third the Km/Vmax value for the galactose-containing substrate for that alpha -sialyltransferase. |
申请公布号 |
AU6827694(A) |
申请公布日期 |
1994.11.21 |
申请号 |
AU19940068276 |
申请日期 |
1994.05.04 |
申请人 |
THE SCRIPPS RESEARCH INSTITUTE |
发明人 |
CHI-HUEY WONG;FEDERICO C.A. GAETA |
分类号 |
C12P19/14;C12N9/10;C12N9/38;C12P19/18;C12P19/26 |
主分类号 |
C12P19/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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