发明名称 |
ZYMOGENIC NUCLEIC ACID DETECTION METHODS, AND RELATED MOLECULES AND KITS |
摘要 |
This application provides methods of detecting and quantitatively determining a target nucleic acid sequence in a sample, which comprise contacting the sample with a primer and a zymogene which encodes, but which itself is the anti-sense sequence of, a catalytic nucleic acid sequence, so that when the target is present, a single amplified nucleic acid molecule is produced which comprises the sequences of both the target and catalytic molecules. This invention further provides a method of simultaneously detecting the presence of a plurality of target nucleic acid sequences in a sample. Finally, this invention provides molecules and kits for practicing the instant methods.
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申请公布号 |
CA2321948(A1) |
申请公布日期 |
1999.09.10 |
申请号 |
CA19992321948 |
申请日期 |
1999.02.22 |
申请人 |
JOHNSON & JOHNSON RESEARCH PTY. LIMITED |
发明人 |
FUERY, CAROLINE J.;CAIRNS, MURRAY J.;TODD, ALISON V. |
分类号 |
C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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