摘要 |
A method of detecting and/or quantifying an IgE antibody specific to a ligand in the form of antigen, antibody or hapten in a liquid sample comprising the steps of (a) contacting (i) the sample with (ii) a ligand in the form of an antigen, an antibody or a hapten to form a mixture (I) comprisng IgE-containing complexes, (b) mixing mixture (I) with a carrier to which is bound (iii) IgE receptor, said IgE receptor being CD23 (Fc epsilon RII) and/or Fc epsilon RI, to form a mixture (II) comprising carrier-bound IgE-containing complexes, (c) separating the carrier-bound IgE-containing complexes from mixture (II) and (d) determining the amount of the carrier-bound IgE-containing complexes formed.
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