发明名称 |
ENONE REDUCTASE GENE AND MICROBIAL PRODUCTION OF LEVODIONE |
摘要 |
Disclosed is an isolated DNA comprising a nucleotide sequence coding for an enzyme having enone reductase activity wherein the enzyme is characterized by the following physico-chemical properties: (a) molecular mass: 61,300±5,000 Da (estimated using gel filtration, consisting of one subunit); (b) co-factor: NADPH and NADH; (c) substrate specificity: active on alpha,beta-unsaturated ketons; (d) optimum temperature: 55-60° C. at pH 7.4; and (e) optimum pH: pH 4.5-8.5. |
申请公布号 |
EP1543125(A2) |
申请公布日期 |
2005.06.22 |
申请号 |
EP20030757875 |
申请日期 |
2003.09.19 |
申请人 |
DSM IP ASSETS B.V. |
发明人 |
KATAOKA, MICHIHIKO;SHIMIZU, SAKAYU |
分类号 |
C12N9/02;C12N15/53;C12P7/26 |
主分类号 |
C12N9/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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