TRANSFECTION SYSTEM FOR PRODUCTION OF TRANSGENIC ANIMAL

摘要

The present invention relates to a transfection system for producing transgenic animals. With the transfection system according to the present invention, a viral vector can be used in order to effect direct genetic modification in an adult animal, such that not only is it possible to cell-specifically regulate inhibition of expression of a target gene by changing solely the cell-specific promoter sequence of the vector and the shRNA sequence of the target gene but it is also possible to inhibit gene expression at a desired time by including a stop codon between two sequences recognized by a site-specific recombination enzyme. Therefore, it is expected that the transfection system according to the present invention can be easily used in studies for the roles of diverse genes as the transfection system is capable of cell-specifically inhibiting the expression of a target gene at a desired time in various species of adult animals when being used.