| 摘要 |
PROBLEM TO BE SOLVED: To relate to a reversibly modified 'hot start' RNase H enzyme composition for the improved CATACLEAVE (TM) probe detection of nucleic acid sequences in a test sample, in which enzyme composition is mainly characterized in that it has the ability to regulate the catalytic activity of the RNase H during the course of a reverse transcription-PCR cycle; thus, RNase H activity can be initially suppressed to minimize degradation of RNA:DNA primer heteroduplexes prior to reverse transcription.SOLUTION: After cDNA synthesis is complete, RNase H activity is induced to promote the cleavage and fluorescent detection of CATACLEAVE (TM) probes that anneal to target DNA sequences within the reverse transcriptase-PCR products. The inducible RNase H enzyme is amenable to high throughput analysis requiring one step reverse transcriptase CATACLEAVE (TM) PCR in a single reaction mix.SELECTED DRAWING: None |
